Technology
How Engineered Heart Muscle Patches Are Generated
The Cell-line From Which The Heart Tissue Is Generated
The iPSC line has been generated from hematopoietic stem cells that were isolated from a cord blood donation and subsequently re-programmed using a standard Yamanaka protocol | 1 |.
A Master Cell Bank and a Working Cell Bank were generated | 2 | and extensively tested to confirm that the line complies with all regulatory requirements | 3 |
How We Generate Our Product
Repairon’s heart muscle tissue is based on an induced pluripotent stem cell (iPSC) line, from which in a first step, cardiomyocytes and stromal cells are generated | 1 | 2 | In a second step these cells are formulated in a collagen hydrogel to support their self-organization into force-generating Engineered Heart Muscle (EHM) for a use as Biological Ventricular Assist Tissue (BioVAT) | 3 |
Functional Cardiomyocytes & Heart Tissue
Spontaneously beating Cardiomyocyte cluster
Spontaneously contracting EHM patch